Principle

In this step, we cluster the cells within each cell type again to separate the doublets as outliers. In Some clusters, like cluster 0 and 1, doublets does not form a unique isolated cluster while others do. We remove those doublet minor populations to enhance the doublet removal performance.

cluster 2

cluster 3

cluster 4

cluster 5

cluster 6

cluster 14

total

Save clean expression profiles for downstream analysis